Nuclear Magnetic Resonance

CIF has variety of NMRs. Varian Mercury-Inova 300 for routine 1H, 13C, 19F, 31P, and Varian Inova 300, 400 and 500 instruments equipped with pulsed field gradients, VT, and a variety of probes for doing nearly any high resolution nmr method and micro-imaging (at 500 MHz). The undergraduate laboratories are equipped with a Varian Gemini2000 broadband 300 MHz NMR for instruction.

Varian Inova 300 1997, dual full-band (1H to 109Ag), 5mm Quad-Nuclear plus X PFG probe, variable temperature, z-axis pulsed-field gradients (PFG), serves broadband users, extended time projects, and walk-up users.
Varian Mercury-Inova 300 (a.k.a. JS-300 NMR) 1997, 1H, 19F, 13C, 31P only, Quad-nucleus PFG probe, variable temperature control, Instrument is HEAVILY used and serveswalk-up users, for routine analysis.
Varian Inova 400 1998, dual full-band (1H to 103Rh), 5mm Quad-Nuclear plus X PFG and 5mm Inverse PFG probes, variable temperature, z-axis pulsed-field gradients (PFG), serves broadband users, extended time projects.
Varian Inova 500 1998, dual full-band (1H to 103Rh) plus third broadband (31P to 103Rh) channel, two waveform generators, 5mm HCN 3-axis PFG, 5mm switchable-broadband, 5mm Inverse PFG probe (Nalorac), 10mm broadband probes, variable temperature, 3-axis pulsed-field gradients (PFG). This instrument is devoted primarily to problems in structural biochemistry and chemical dynamics.

Varian Gemini-2000 BB

1992, upgraded to model-2000 during fall '97. Purchased by the University for student undergraduate laboratory use.

Some of the Experimental Methods Available in CIF: Most on-campus users opt for individual training on the various instruments and then do their own work. We can provide complete spectral servicesfor anyone needing it but this is, of course, more expensive.

Training:Most user training begins on the JS-300 and includes a formal checkout on the safe use of the equipment. Since many of the most popular NMR methods (one and two-dimensional) are available by accessing the Varian software menus, there is little formal training at this level. Users are given their own accounts on the system. Classes in advanced methods are offered periodically.

Routine Proton, Fluorine, Phosphorus, and Carbon NMR: The JS-300 (Mercury-Inova) is dedicated to this effort, however, all the instruments have this capability built in.

Broad-Band: This is a general category of experiments that involves detection of nucleithat are not 1H, 13C. 31P, or 19F. With the exception of the JS-300, all of the Inova spectrometers are equipped to observe broadband nuclei.

Advanced 1D experiments: Experiments that are readily available in the CIF include 1D versions of homonuclear NOE, PFG experiments, various gated de-coupling experiments including those with polarization transfer, X nucleus de-coupling, high-band/low-band crossings, T1 and T2 measurements and selective irradiation or inversion experiments. These experiments are available on all instruments in the lab through the software menu system. There are many other experiments which can be done after receiving some assistance or training from lab staff.

Two-D NMR: Basic 1H-1H homonuclear two-D experiments are available on all spectrometers. COSY, HetCor, Jres, Noesy, Roesy, Exsy, Tocsy, etc. Most of these are available through the menu system. Let us know if something is missing.

Advanced NMR Methods: You know what you want to try. Come discuss these experiments with us and we will get you going.

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